产虾青素的斜生栅藻藻种的培育

摘 要 ：考察了亚硒酸钠浓度对斜生栅藻（Scenedeus obliquus）生物量的影响以及2.0 g/L亚硝基胍和不同紫外辐射照度胁迫对斜生栅藻中虾青素含量的影响.结果表明,斜生栅藻生物量随着亚硒酸钠处理浓度的增加而增加,其藻内虾青素含量随着紫外辐射照度的增加而增加,关 键 词 ：斜生栅藻（Scenedeus obliquus）；生物量；育种；虾青素含量

中图分类号：Q945.1 文献标识码：A 文章编号：0439-8114（2014）02-0283-02

Cultivation of Algal Species of Scenedeus obliquus Which Producing Astaxantin

LING Shan-feng

（College of Biological engineering, Jingchu University of Technology,Jingmen 448000,Hubei,China）

Abstract： Effects of concentration of sodium selenite on Scenedeus obliquus biomass were studied. Effects of 2.0 g/L NTG and UV irradiance stress on onastaxanthin content of S. obliquus was investigated. The results showed that the biomass of Scenedeus obliquus increased when the concentration of sodium selenite increased. The astaxanthin content of Scenedeus obliquus increased when the UV-B irradiation dose of Scenedeus obliquus increased. When UV-B irradiation was 0.6 W/m2,the astaxanthin content of Scenedeus obliquus in the bined treatment group was 37.15 times higher than that of the control with the significant difference（P<0.05）.

Key words： Scenedeus obliquus； biomass； breeding； astaxanthin content

细胞在48 h内生成并积累了虾青素.肖媛等[2]报道了紫外辐射5 min 后可使雨生红球藻细胞内的虾青素含量显著提高.此外,紫外线、激光复合诱变也可使雨生红球藻中的虾青素含量显著提高,而且紫外线诱变株能在非高光胁迫条件下快速积累色素,表明突变株对光的敏感程度提高[8,9].有“超级诱变剂”之称的亚硝基胍是光合作用氧生成反应中电子传递的抑制剂[10],曾被成功用来诱导雨生红球藻建立突变体,浓度为2.5 g/L的亚硝基胍是诱导雨生红球藻积累虾青素的最适诱导剂量[11].本试验研究结果表明,在2.0 g/L的亚硝基胍胁迫下,斜生栅藻藻体内虾青素含量随着紫外辐射照度的增加而增加,0.6 W/m2紫外辐射照度使斜生栅藻内虾青素含量比辐射照度为0提高37.15倍.由此说明紫外辐射和亚硝基胍组合胁迫是一种非常有效的方法,为下一步深入研究其机理奠定了基础.

致谢：特别感谢蔡福欢、刘彦文、朱勇、韩梁、程淼同学在试验过程中付出的辛勤劳动,以及朱德艳老师,袁启力、张盛仁书记的协助.